全长的nsp2复制酶有助于高度致病性PRRSV-2中的病毒聚集
Yuan-Zhe Bai1, Shujie Wang1, Yue Sun1
1State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China.
Journal of virology
|November 27, 2024
概括
高致病性猪生殖和呼吸系统综合征病毒2型 (HP-PRRSV-2) 组合涉及非结构蛋白2 (nsp2),这促进了核体 (N) 蛋白与ER和ERGIC中的病毒包膜蛋白的包装. 这种相互作用对于病毒组合至关重要.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 动物健康 动物健康
背景情况:
- 猪生殖和呼吸系统综合征病毒 (PRRSV) 对全球猪业产生重大影响.
- PRRSV病毒的组装机制,特别是核体 (N) 蛋白和病毒包膜蛋白之间的相互作用,尚不清楚.
研究的目的:
- 阐明非结构蛋白2 (nsp2) 在高度致病性PRRSV-2 (HP-PRRSV-2) 病毒组合中的作用.
- 在组装过程中调查nsp2,N蛋白和病毒包膜蛋白之间的相互作用.
主要方法:
- 共同免疫沉 (Co-IP) 试验被用来分析蛋白质与蛋白质之间的相互作用.
- 实验涉及nsp2,N蛋白和病毒包膜蛋白的全长和截断形式 (M,GP2a,GP5).
- 亚细胞局部化研究是在内质网膜 (ER) 和ER-Golgi中间体 (ERGIC) 中进行的.
主要成果:
- 全长的nsp2,但没有截断的形式 (nsp2TF,nsp2N),与N蛋白和病毒包膜蛋白 (M,GP2a,GP5) 共同免疫.
- nsp2促进N蛋白和病毒包膜蛋白之间的相互作用.
- 这些相互作用和组装过程发生在ER和ERGIC内部.
结论:
- 全长的nsp2通过调解N蛋白和病毒包膜蛋白之间的相互作用,在HP-PRRSV-2的组装中发挥关键作用.
- 这项研究为病毒组装过程提供了新的见解,并为针对PRRSV-2的抗病毒策略提供了潜在的目标.
相关概念视频
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
01:29Viruses with RNA Genomes
3
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
3
03:01The Replisome
33.0K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.0K
02:36RNA Polymerase II Accessory Proteins
9.1K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.1K
02:41Protein Complex Assembly
10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
01:10Retrovirus Life Cycles
45.7K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.7K