细菌被设计为产生血清素,调节宿主肠道生理学
Chrystal F Mavros1,2, Mareike Bongers3, Frederik B F Neergaard3
1Department of Systems Biology, Columbia University Irving Medical Center, New York, New York 10032, United States.
ACS synthetic biology
|November 27, 2024
概括
工程化肠道细菌Escherichia coli Nissle 1917被开发用于在小鼠中产生血清素. 这种微生物疗法调节了肠道生理和基因表达,为治疗人类肠道疾病提供了潜力.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 代谢工程是代谢工程.
背景情况:
- 肠道细菌显著影响宿主肠道功能,包括运动性和恒常性.
- 微生物代谢物和宿主生理学之间的复杂相互作用在理解肠道健康和疾病方面提出了挑战.
- 由肠道微生物产生的生物氨基酸是宿主微生物相互作用的关键媒介.
研究的目的:
- 为了设计一种肠道共生细菌, *Escherichia coli* Nissle 1917,用于人类代谢物血清素的生物合成.
- 为了研究微生物产生的血清素对宿主胃肠道的生理影响.
- 用工程细菌建立一个用于调节光血清水平的新框架.
主要方法:
- 基因工程的 * Escherichia coli * 尼斯尔1917年,以使血清素的生产.
- 将工程细菌口服给一个小鼠模型.
- 对治疗后宿主肠道转录和生理变化的分析.
主要成果:
- 工程细菌成功地殖民大肠,并在体内产生血清素.
- 用产生血清素的细菌治疗的小鼠在肠道中表现出显著的生物学变化.
- 观察到的变化包括肠道水平的转录和生理修饰.
结论:
- 工程细菌提供了一个可行的平台,用于调节肠道中的光线血清素水平.
- 微生物产生的血清影响宿主肠道生理学,提供了对肠道平衡和功能障碍的见解.
- 这种方法对开发人类胃肠道疾病的新型微生物疗法具有前景.
相关概念视频
01:29Bacterial Flora of the Large Intestine
385
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
385
01:29Microorganisms in Medicine and Therapeutics
1
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1
01:28Gene Regulation in Microbial Communities: Quorum Sensing
3
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
3
01:50What is Monogastric Digestion?
70.6K
The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. Several other animal species also have monogastric digestive systems, including pigs, horses, dogs, and birds. This chapter, however, focuses on the human digestive system.
70.6K