进化非酸化代谢以改善二氧格卢酸衍生物的生产
Jing Zhao1, Jilong Wang2, Jingyu Wang1
1School of Engineering, Westlake University, Hangzhou 310030, P. R. China.
Journal of agricultural and food chemistry
|November 27, 2024
概括
设计了非酸化代谢中的一个关键酶,以改善从林氏纤维素生物质中生产的2-氧格酸盐 (2-KG) 衍生物的生产. 这增强了d-西洛斯转化为有价值的化合物,如l-谷氨酸和l-.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 生物质转换生物质转换
背景情况:
- 基纤维素生物质为增值产品提供了一个可持续的资源.
- 非酸化的新陈代谢有效地将丁和d-银酸盐转化为2-氧格酸盐 (2-KG).
- 在Caulobacter crescentus中,韦姆伯格通路受到低CcXylX活性的限制.
研究的目的:
- 为了提高2-KG衍生物的生产,从林氏纤维素生物质.
- 为了克服CcXylX的低催化效率,CcXylX是维姆伯格路径中的关键酶.
- 提高从d-xylose生产2-KG衍生物的经济可行性.
主要方法:
- 使用定向进化来设计CcXylX酶.
- 工程CcXylX突变的催化活性 (kcat) 的表征.
- 使用d-西洛斯作为基质生产2-KG衍生物的发酵研究.
主要成果:
- 与野生类型相比,工程CcXylX突变体显示了3倍高的Kcat.
- 增加了32% (8.3 g/L) 的l-谷氨酸和79% (4.3 g/L) 的l-林的产量.
- 证明了从d-xylose,一个lignocellulosic成分中获得改善的2-KG衍生物产量.
结论:
- 酶工程可以显著改善非酸化代谢途径.
- 这项工作推进了用于可持续化学品生产的纤维纤维素生物技术.
- 为经济可行的生产各种2-KG衍生品提供基础.
相关概念视频
Other Glycolytic Pathways
1
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
1
Drug Metabolism: Phase II Reactions
3.7K
Phase II reactions are essential for the detoxification and elimination of drugs from the body. These reactions involve the conjugation of parent drugs or their phase I metabolites with endogenous molecules, resulting in more hydrophilic drug conjugates. The primary conjugation reactions in this phase are sulfation and glucuronidation. Both sulfation and glucuronidation typically produce biologically inactive metabolites. However, in some cases involving prodrugs, active metabolites may be...
3.7K
Amino Acid Biosynthetic Pathways
3
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
3
Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
146
Glutathione, a tripeptide made up of glutamate, cysteine, and glycine, is a critical player in the detoxification of drugs and xenobiotics via a process known as glutathione conjugation or mercapturic acid formation. This phase II biotransformation reaction involves the covalent binding of glutathione to a drug or its metabolite, enhancing the compound's water solubility and enabling its excretion.
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
146
Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
Inorganic Nitrogen Assimilation
5
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
5


