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一个多功能DNA纳米组件用于癌细胞检测和组合基因化学疗法治疗

Ruyan Zhang¹, Ting Zhou¹, Xiaobing Huo¹

¹College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.

Langmuir : the ACS journal of surfaces and colloids

|November 27, 2024

概括

这项研究引入了用于增强癌症治疗的DNA纳米组件 (RAD). 雷达向癌细胞,为基因治疗提供DNAzyme和化疗提供多克索鲁比,提高治疗效率和稳定性.

科学领域:

生物技术是生物技术.
纳米医学是一种纳米医学.
癌症治疗癌症治疗

背景情况:

基于DNA酶的基因疗法面临着诸如细胞吸收率低和稳定性差等挑战.
使用DNAzyme或化疗剂的单一治疗往往在癌症治疗中产生不满意的结果.

研究的目的:

开发一种多功能DNA纳米组件 (RAD),以改善癌细胞检测和DNAzyme和多克索鲁比 (DOX) 的有针对性的联合递送.
通过结合基因疗法和化疗来增强癌症治疗的疗效.

主要方法:

使用滚动圆放大 (RCA) 产品作为支架构建的RAD,结合了APTAMER AS1411,光组和DNA酶序列.
利用多价值的阿巴 - 核素相互作用,用于选择性细胞吸收和细胞内输送DOX和DNAzyme.
评估RAD对HepG-2细胞检测的敏感性及其体外治疗效果.

主要成果:

RAD对 HepG-2 细胞检测 (LOD 200 细胞/毫升) 和高多克索鲁比辛负载能力 (每 RAD 最大 200 个 DOX 分子) 显示出高灵敏度.
RAD有效地和选择性地将DOX和DNAzyme输送到癌细胞中,通过DNAzyme介导的幸存mRNA裂变和通过DOX的化疗,导致癌细胞亡.
结合疗法 (RAD-DOX) 与单一疗法相比显示出增强的治疗效果,RAD为正常细胞提供保护.

结论:

多功能DNA纳米组件 (RAD) 提供增强的血清稳定性,高药物载荷能力和选择性的细胞吸收.
通过RAD介导的DNAzyme和DOX的同时递送为有效的癌症基因疗法和化疗提供了一个有希望的策略.
这种方法在推进癌症治疗方式方面具有重大潜力.

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