对通过游泳回收的精子的影响
Melina Faggi1, Cecilia Paparella1,2, Patricia Perfumo1,2,3
1Laboratorio de Medicina Reproductiva. Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina.
Journal of assisted reproduction and genetics
|November 27, 2024
概括
在精子上游过程中补充可以显著减少DNA碎片化,改善精子质量. 这种方法增强了用于辅助生殖技术 (ART) 的精子选择,提供了更好的结果.
科学领域:
- 生殖生物学 生殖生物学
- 精子生理学 精子生理学
- 辅助生殖技术 辅助生殖技术
背景情况:
- 影响精子染色质的稳定性,运动性和氧化应激保护.
- 精子游泳是一种标准的辅助生殖技术 (ART) 程序.
- 人们对在上游过程中精子DNA碎片化存在担忧.
研究的目的:
- 调查精子游泳期间补充剂对精子质量的影响.
- 评估在缓解DNA碎片化和增强精子参数方面的作用.
主要方法:
- 分析了来自203名诺莫动物种子男性的精液样本.
- 用人管液 (mHTF) 用不同度或没有补充 (对照) 的精子进行了上游.
- 评估的参数包括DNA碎片化,染色质成熟度,ROS水平,运动性和蛋白质酸化.
主要成果:
- 补充剂在所有测试度 (p < 0.0001) 中显著降低了精子DNA碎片化.
- 通过2.5和3.5毫米观察到更好的染色质成熟度.
- 特定运动性参数得到了2.5mM (速度,节拍交叉频率) 和5mM (直度,线性,超运动性) 的改善. 随着5毫米的使用,ROS水平下降.
结论:
- 将添加到mHTF中,可以在上游过程中为精子创造最佳条件.
- 补充剂是改善精子选择在ART中的一个有价值的策略.
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