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使用全面的在线UHPLC×UHPSFC/Tandem质谱法进行四维脂质组分析

Zuzana Lásko¹, Tomáš Hájek¹, Robert Jirásko¹

¹Department of Analytical Chemistry, University of Pardubice, Faculty of Chemical Technology, Studentská 573, Pardubice 53210, Czech Republic.

Analytical chemistry

|November 27, 2024

概括

这项研究引入了一种新的多维染色学方法用于脂质组分析,显著增加了复杂生物样本的峰值容量. 这种先进的技术使得详细的脂体表征和识别许多脂物种成为可能.

科学领域:

分析化学分析化学
生物化学生物化学
染色体学染色体学是一种染色学.

背景情况:

复杂的生物样本需要先进的分离技术进行全面分析.
多维色谱可以提高分辨率和峰值容量,这对脂管学至关重要.
现有的方法可能缺乏详细的脂质组分析所需的分辨率.

研究的目的:

开发一种新的全面的在线多维染色学方法,用于脂质组分析.
将逆相超高性能液态染色学 (RP-UHPLC) 和超高性能超临界流体染色学 (UHPSFC) 结合起来,以增强分离.
显著增加生物样本中脂类物种详细表征的峰值能力.

主要方法:

一个二维色谱系统,将RP-UHPLC (150毫米C18柱) 和UHPSFC (10毫米柱) 与2微米以下的颗粒相结合.
将系统与使用电喷射电离 (ESI) 的高分辨率四极飞行时间 (QTOF) 质量分析仪相合.
使用正极和负极的全扫描和双重质谱 (MS/MS),包括MS^E和快速依赖数据的获取.

主要成果:

它的峰值容量是RP-UHPLC的10倍,是单独的UHPSFC的18倍.
启用了第二维超快速分析 (UHPSFC),采样时间为0.55分钟.
成功地描述了人体血的脂质组概况,在16个子类中确定了298种脂质物种,具有高可靠性.

结论:

开发的多维染色学方法为脂质组分析提供了卓越的峰值容量和分辨率.
这种方法允许详细表征和自信识别广泛的脂类物种.
该方法有效用于分析复杂的生物矩阵,如人体血.

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