对TRPC蛋白在SOCE,ROCE和CRAC通道功能的参与有遗传证据
Sebastian Susperreguy1, Megumi Yamashita2, Chan-Il Choi3
1Institute of Biomedical Research, School of Biomedical Sciences, Catholic University of Argentina, Buenos Aires C1107AFF, Argentina.
概括
储量耗尽激活进入通道 (SOCE) 依赖于Orai1,而不是TRPC分子. 缺少所有TRPC基因的TRPC heptaKO小鼠是可行的和肥沃的,从而开辟了新的研究途径.
科学领域:
- 细胞生物学 细胞生物学
- 分子生理学分子生理学
- 离子通道功能的功能
背景情况:
- 储存运行的入 (SOCE) 对于细胞信号传输至关重要.
- 和TRPC蛋白是通道的已知组成部分,但它们在SOCE中的确切作用仍在争论中.
研究的目的:
- 调查Orai和TRPC蛋白在SOCE和受体操作入 (ROCE) 中的强制性作用.
- 为了表征TRPC heptaKO小鼠和缺乏所有七个TRPC基因的细胞.
主要方法:
- 使用了基因工程小鼠和衍生细胞系 (TRPC heptaKO).
- 采用Fura2染料来监测的进入.
- 对释放激活电流 (Icrac) 进行了生物物理测量.
主要成果:
- 对于SOCE来说,Orai1是必不可少的,在Orai2和Orai3上表现出主导地位.
- 由于缺乏功能TRPC蛋白质,SOCE和Icrac不受影响.
- 独立于TRPC的ROCE也需要Orai1.1.
- TRPC heptaKO小鼠是肥沃和可行的.
结论:
- 在SOCE中,Orai1,而不是TRPC蛋白,是强制参与的.
- 此外,Orai1还通过TRPC独立的ROCE进行调解.
- TRPC heptaKO小鼠为研究信号通路提供了有价值的模型.
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