基于SERS的Aptamer传感策略用于糖尿病生物标志物检测
Jihong Wang1, Shuang Guo2, Eungyeong Park3
1State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China.
Analytical chemistry
|November 27, 2024
概括
这项研究引入了一种新的表面增强拉曼散射 (SERS) 适应传感器,用于检测葡萄糖和胰岛素. 开发的SERS口腔传感器在唾液中检测疾病生物标志物的高度选择性和灵敏性.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 纳米技术 纳米技术
背景情况:
- 准确的葡萄糖和胰岛素检测对于糖尿病诊断和管理至关重要.
- 目前的检测方法可能缺乏早期疾病识别所需的灵敏度或特异性.
- 糖尿病影响全球数百万人,需要先进的诊断工具.
研究的目的:
- 开发一种新的表面增强拉曼散射 (SERS) 适应传感器,用于同时检测葡萄糖和胰岛素.
- 为了提高灵敏度,利用金双金字塔纳米颗粒 (Au BPs) 作为SERS基质.
- 为了验证口味传感器在真实世界生物样本 (如唾液) 中的性能.
主要方法:
- 使用金双纳米颗粒 (Au BPs),SH-aptamer-methylene blue (SH-aptamer-MB) 和硫化聚乙烯甘醇 (SH-PEG) 制造SERS吸收传感器.
- 通过Au-S债券将SH-aptamer-MB固定在Au BPs上,而MB则担任拉曼报告员.
- 在葡萄糖和胰岛素结合后,利用aptamer的构造变化来调节MB的SERS信号.
主要成果:
- 对于敏感的SERS检测,Au BPs提供了丰富的"热点".
- 胃感应器显示出与葡萄糖和胰岛素的特定结合,导致MB SERS信号的可检测变化.
- 使用唾液样本成功验证了口味传感器,对选择性和灵敏性产生了令人满意的结果.
结论:
- 开发的 SERS 胃感应器为准确检测葡萄糖和胰岛素提供了一个有前途的平台.
- 这种方法为利用SERS在检测疾病生物标志物的过程中提供了一种有效的策略.
- 胃感应器的高选择性和可靠的灵敏度为改善糖尿病诊断铺平了道路.
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