基于凝的颗粒凝作为生物制造的悬浮介质
Andrew McCormack1, Laura M Porcza1, Nicholas R Leslie1
1Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, United Kingdom.
PloS one
|November 27, 2024
概括
研究人员开发了剪切诱导的凝糖颗粒凝作为3D生物打印的新型悬浮介质. 这些凝可以在各种温度下高速打印仿生结构,从而推进再生医学应用.
科学领域:
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
- 再生医学是一种再生医学.
背景情况:
- 3D组织工程在创造仿生结构方面面临着挑战.
- 粘性塑料液体正在成为先进生物墨水的悬浮介质.
- 格兰颗粒状凝具有作为一种新的生物制造介质的潜力.
研究的目的:
- 为3D生物打印提供剪切诱导的凝颗粒凝作为悬浮介质.
- 为了评估这些颗粒状凝的粘性质和可打印性.
- 为了证明细胞培养和制造可交叉链接的水凝特性的潜力.
主要方法:
- 在冷却诱导的凝过程中通过剪切产生凝糖颗粒凝.
- 在温度范围内的颗粒状凝粘性质的表征.
- 使用颗粒状凝悬浮剂3D生物打印纤维和带有细胞的液滴.
- 在7天内评估细胞活力和培养.
- 在可交叉连接的悬浮介质中制造水凝特征.
主要成果:
- 格兰颗粒型凝在广泛的温度范围内 (从4°C到生理温度) 呈现粘性可塑性.
- 低产压力 (低至0.4Pa) 能够实现高速打印 (高达60mm/s).
- 成功打印和维护7天内载有细胞的液滴.
- 制造水凝的特点在一个可交叉连接的凝和凝-甲基烯酸 suspension.
结论:
- 剪切诱导的凝颗粒凝是一种用于3D生物打印的多功能悬浮介质.
- 这些材料的特性促进了高速,多温度的生物制造.
- 这种方法对先进的组织工程和再生医学应用有希望.
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