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相关概念视频

Cohesins02:20

Cohesins

4.4K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.4K
Condensins02:15

Condensins

3.4K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.4K
Nucleosome Remodeling02:54

Nucleosome Remodeling

9.0K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.0K
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

3.3K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
3.3K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

3.2K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
3.2K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

3.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K

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相关实验视频

Updated: Jun 6, 2025

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
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Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

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SMC介导的染色体组织:循环挤出能解释一切吗?

Tatsuya Hirano1, Kazuhisa Kinoshita1

  • 1Chromosome Dynamics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Current opinion in cell biology
|November 27, 2024
PubMed
概括

循环挤出是通过对染色体 (SMC) 蛋白质的结构维护来进行染色体组织的一个关键模型. 本综述探讨了超越循环挤出的替代SMC机制,澄清了染色体生物学中尚未解决的问题.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 细胞生物学 细胞生物学

背景情况:

  • 染色体 (SMC) 蛋白质复合体的结构维护,包括凝聚素和凝聚素,对于染色体组织至关重要.
  • 循环挤出是SMC介导的染色体组织的一个突出提出的机制.
  • 循环挤出的精确生理相关性和操作仍在调查中.

研究的目的:

  • 审查拟议的染色体组织的非循环挤出机制.
  • 为了澄清有关SMC蛋白质复合体功能的未解决问题.
  • 为了全面了解SMC复合体在细胞中如何运作.

主要方法:

  • 对拟议的非循环挤出模型的文献综述.
  • 对替代性SMC介导机制的现有证据的分析.
  • 综合当前的知识和识别研究差距.

主要成果:

  • 在科学文献中提出了几种用于染色体组织的非循环挤出机制.
  • 循环挤出 in vivo 功能的确切条件和程度尚未完全确定.
  • 除了简单的循环挤出之外,SMC复合物的替代或额外的作用是合理的.

更多相关视频

CRISPR-Mediated Reorganization of Chromatin Loop Structure
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CRISPR-Mediated Reorganization of Chromatin Loop Structure

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Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
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Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

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相关实验视频

Last Updated: Jun 6, 2025

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

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CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

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Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

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结论:

  • 要了解SMC复合体的多样性功能,需要考虑超越循环挤压的机制.
  • 需要进一步的研究来阐明SMC介导的染色体组织策略的完整目录.
  • 澄清这些机制对于全面了解基因组结构和功能至关重要.