无处不在的pyridoxal 5'-酸盐结合蛋白也是一个RNA结合蛋白质
Claudio Graziani1,2, Anna Barile3, Alessia Parroni3
1Dipartimento di Scienze Biochimiche "A. Rossi Fanelli", Sapienza Università di Roma, Rome, Italy.
Protein science : a publication of the Protein Society
|November 28, 2024
概括
皮里多克萨尔5'-酸盐结合蛋白 (PLP-BP) 结合RNA,结合强度由PLP调节. 这种涉及lys137的保守相互作用表明它在细胞调节中的作用.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 皮里多克萨尔5'-酸盐结合蛋白 (PLP-BP) 对于所有生命王国的PLP稳态至关重要.
- 人类PLP-BP变种与新生儿有关,但它的确切功能仍然未知.
- 大肠杆菌YggS是一种经过充分研究的PLP-BP同源体.
研究的目的:
- 阐明PLP-BP在细胞代谢中的功能.
- 为了研究YggS和RNA之间的相互作用.
- 确定PLP在调节这种相互作用中的作用.
主要方法:
- 通过YggS研究RNA结合.
- 分析了PLP对RNA结合亲和力的影响.
- 利用RNA-seq来识别共同净化的RNA.
- 检查了RNA结合部位的进化保护.
主要成果:
- YggS直接与RNA结合,PLP调节这种相互作用.
- lys137对于RNA结合至关重要.
- 人类的PLP-BP中保留了RNA结合部位.
- 鉴定出SsrA和RnpBRNA是主要的结合伙伴.
- 通过改变蛋白质构成,PLP可能会影响RNA结合亲和力.
结论:
- PLP-BP的功能可能涉及由PLP调节的RNA相互作用.
- 这种相互作用表明在一个不具特征的监管机制中发挥了作用.
- 这些发现为了解PLP-BP在细胞过程中的作用开辟了新的途径.
相关概念视频
Bacterial RNA Polymerase
28.8K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
28.8K
Nucleic Acids
43.7K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
43.7K
Nucleic acids
160.0K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
160.0K
Types of RNA
63.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.3K
Ribosomal RNA Synthesis
13.1K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.1K
RNA Stability
33.3K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.3K


