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解读硬小麦酸盐过氧化酶TdAPX7B-2在非生物应激反应中的作用

Kaouthar Feki¹, Sana Tounsi^2,3, Hanen Kamoun²

¹Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS), BP1177, 3018, Sfax, Tunisia. kaouther.feki@cbs.rnrt.tn.

Functional & integrative genomics

|November 28, 2024

概括

硬小麦亚酸盐过氧化酶 (APX) 基因TdAPX和TdAPX-R被保存并显示净化选择. TdAPX7B-2基因通过调节抗氧化酶和蛋白积累,增强了对环境压力的耐受性.

科学领域:

植物分子生物学植物分子生物学
生物化学生物化学
基因组学就是基因组学.

背景情况:

在氧化应激过程中,酸盐过氧化酶 (APX) 酶在氧化应激过程中清除过氧化 (H2O2).
硬小麦 (Triticum durum) 具有APX和相关APX-R蛋白的家族.

研究的目的:

在 durum小麦中对TdAPX和TdAPX-R基因家族进行全基因组分析.
为了功能性地描述TdAPX7B-2基因及其在耐压力中的作用.

主要方法:

全基因组分析,包括外子-内子结构,合成,保存动机和多个序列对齐.
用RNA测序 (RNA-seq) 和定量逆转录PCR (qRT-PCR) 进行基因表达分析.
在各种压力条件下 (盐,寒冷,干旱,金属,ABA) 使用表达TdAPX7B-2的Arabidopsis thaliana进行功能性表征.

主要成果:

TdAPX和TdAPX-R基因被分为四个子组,在进化过程中相对保持,经历净化选择和细分重复.
活性部位残留物仅在TdAPX蛋白中保存.
在盐,寒冷,干旱,金属和ABA处理下,TdAPX7B-2在 durum小麦叶中得到上调.
表达TdAPX7B-2的转基因阿拉比多普西斯菌株表现出对盐,氧化应激和重金属的增强耐受性,由根部发育,烯积累和抗氧化酶 (CAT,SOD,POD) 的诱导证明.
转基因系中的应激反应涉及引发关键的应激基因,如AtNCED3,AtRD29A/B和AtERD1.

结论:

durum小麦中的TdAPX和TdAPX-R基因家族表现出保存的进化模式.
通过调节抗氧化剂防御机制和透调整,TdAPX7B-2在增强植物对多种非生物应激的耐受性方面发挥着重要作用.

关键词:

阿斯科巴特过氧化酶的使用.硬小麦硬小麦是一种硬小麦.进行全基因组分析.重金属是一种重金属.氧化应激是一种氧化应激.ROS的新陈代谢过程

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