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Updated: Jun 22, 2026

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在长期存储的组织的光和电子显微镜:从老鼠肝脏样本学到的教训

Gerald J Shami1,2, Zenan Chen3,4, Delfine Cheng4,5

  • 1University of Sydney, School of Medical Sciences (Molecular and Cellular Biomedicine), Sydney, Australia. gerald.shami@sydney.edu.au.

Histochemistry and cell biology
|November 28, 2024
PubMed
概括

鼠肝组织可以在特定的固定剂中长时间储存,从而使光和电子显微镜都成为可能. 最佳的储存条件保持了超结构完整性,并允许可靠的成像和分析.

关键词:
阿尔德化物是什么固定方式 固定方式光灯标签的使用方法多模态显微镜的使用方法组织的保存 组织的保存.

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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...