在活体中,三甲基胺酶表达和糖溶性通路之间的相互作用机制
Qian Li1,2,3, Di Wu1,2, Yu Song1,2
1Tianjin Agricultural University, Tianjin 300392, PR China. zhangmin@tjau.edu.cn.
Food & function
|November 28, 2024
概括
饮食和干预措施影响肠道微生物和代谢物生产. 富含阿拉比诺基兰的饮食和糖溶性抑制剂降低了TMA溶酶,这表明个性化的饮食策略可以控制TMA的产生.
科学领域:
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
- 主体微生物群的相互作用
背景情况:
- 主体-肠道微生物群的相互作用通过TMA溶酶影响与疾病相关的代谢物三甲基胺 (TMA) 的产生.
- 调节微生物酶生产的机制,特别是TMA酶,仍然不完全理解.
研究的目的:
- 研究饮食和干预因素如何影响肠道微生物群,基因表达,以及TMA溶酶和小鼠中的糖解路径之间的相互作用.
- 阐明控制肠道TMA产生的监管机制.
主要方法:
- 利用16S rRNA基因测序,转基因组学和代谢学来分析肠道微生物组成和功能性基因表达.
- 在对饮食和干预改变的反应中,评估了与TMA酶和糖解酶相关的微生物基因表达特征.
主要成果:
- 不同的饮食和干预措施显著改变了肠道微生物群的组成,基因表达和与糖氨酸代谢和糖解相关的代谢物.
- 富含阿拉比诺基兰的饮食抑制了参与胆,甘氨酸,糖解和TMA溶解的基因,通过酸盐路径促进了甘氨酸的利用.
- 糖溶性抑制剂,特别是那些向酸盐激酶的抑制剂,放大了对基因表达和代谢物概况的观察效应.
结论:
- 在调节肠道微生物群中的甘氨酸水平方面,TMA溶酶和甘氨酸溶解途径之间有显著的交叉关系.
- 研究结果表明,有针对性的干预措施和个性化的饮食方法,如添加富含阿拉比诺基兰的食物,可以有效调节TMA酶的产生.
- 突出了通过饮食操纵和了解微生物代谢途径来管理TMA相关的健康风险的潜在策略.
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