Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Investigating the 8CB liquid crystal-insulin interaction: The role of the smectic a phase in enhancing detection sensitivity.

International journal of biological macromolecules·2025
Same author

Proposed biosensor for the detection of Bovine Serum Albumin using 7CB liquid crystals: A preliminary study.

International journal of biological macromolecules·2024
查看所有相关文章

相关实验视频

Updated: Jul 9, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

11.7K

开发一个生物传感原型,使用7CB液晶来检测人类胰岛素.

Athul Satya1, Ayon Bhattacharjee1

  • 1Department of Physics, National Institute of Technology, Bijni Complex, Laitumkhrah, Shillong, Meghalaya 793003, India. ayonbh@nitm.ac.in.

Physical chemistry chemical physics : PCCP
|November 28, 2024
PubMed
概括

这项研究引入了一种新的液晶生物传感器,用于检测人体胰岛素. 4-heptyl-4-biphenylcarbonitrile液晶 (7CB-LC) 原型使无标签的胰岛素检测具有高选择性和低检测极限.

科学领域:

  • 材料科学 材料科学 材料科学
  • 生物化学 生物化学
  • 分析化学 分析化学

背景情况:

  • 人类胰岛素对葡萄糖调节至关重要;其缺乏会导致高血糖症,影响主要器官.
  • 目前的胰岛素检测方法可能缺乏灵敏度或需要标签.
  • 液晶 (LC) 提供了无标签生物传感应用的潜力.

研究的目的:

  • 开发和验证一种新的原型生物传感器,用于无标签检测人类胰岛素.
  • 研究人类胰岛素和4-heptyl-4-biphenylcarbonitrile液晶 (7CB-LC) 之间的相互作用.
  • 为了确定7CB-LC生物传感器对人类胰岛素的敏感性和选择性.

主要方法:

  • 使用了4-heptyl-4-biphenylcarbonitrile液晶 (7CB-LC) 作为传感材料.
  • 使用偏振光学显微镜 (POM) 进行胰岛素-LC相互作用的纹理分析.
  • 进行了RGB/灰色指数研究,分子对接和拉曼光谱学以进行表征.

主要成果:

  • 人类胰岛素在7CB-LC.中诱导了不同的纹理 (辐射,扭曲-辐射,辐射前,双极).
  • 人类胰岛素的检测极限达到25μM.

更多相关视频

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.7K
Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K

相关实验视频

Last Updated: Jul 9, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

11.7K
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.7K
Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

1.9K
  • 在相关性研究中显示出高选择性,R2值为0.97279.
  • 分子对接和拉曼光谱阐明了胰岛素-LC相互作用机制.

    • 结论:

    • 7CB-LC原型显示了无标签人体胰岛素生物感应的巨大潜力.
    • 观察到的纹理变化和光谱数据证实了选择性相互作用.
    • 这种方法为视觉和敏感胰岛素检测提供了一个有希望的方法.