相关实验视频

Updated: Jun 6, 2025

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
10:39

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

Published on: June 12, 2013

13.5K

在细胞间传播过程中,Shigella flexneri需要一个具有转移能力的孔隙才能从双膜真空中逃脱

Julie E Raab, Tucker B Harju, Jody D Toperzer

    bioRxiv : the preprint server for biology
    |November 28, 2024
    PubMed
    概括

    石格拉柔性菌利用其3型分泌系统 (T3SS) 孔蛋白IpaC传递毒性因子,使细菌在宿主细胞之间传播. 这项研究揭示了在传播过程中明显的T3SS调节,这对于逃脱宿主细胞真空非常重要.

    更多相关视频

    Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance10:41

    Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

    Published on: January 3, 2012

    13.3K
    Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells08:56

    Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells

    Published on: May 24, 2018

    5.5K

    相关概念视频

    Bacterial Translocation and Protein Secretion01:26

    Bacterial Translocation and Protein Secretion

    Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
    2
    Gram-negative Bacterial Protein Secretion Systems01:17

    Gram-negative Bacterial Protein Secretion Systems

    Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
    1
    Protein Translocation Machinery on the ER Membrane01:28

    Protein Translocation Machinery on the ER Membrane

    The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
    Sec61 protein conducting channel
    In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
    4.5K
    Cotranslational Protein Translocation01:20

    Cotranslational Protein Translocation

    Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
    Sec61 channel partners for cotranslational translocation
    During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
    7.2K
    Protein Transport into the Inner Mitochondrial Membrane01:34

    Protein Transport into the Inner Mitochondrial Membrane

    Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
    Transport of mitochondrial precursors across the TIM23 channel is driven by...
    3.6K
    Insertion of Single-pass Transmembrane Proteins in the RER01:26

    Insertion of Single-pass Transmembrane Proteins in the RER

    Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
    Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
    6.6K