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Updated: Jun 14, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

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基因编码纳米粒子的单粒子跟踪:优化表达细胞质扩散研究的表达.

Elizaveta Korunova1, Vitali Sikirzhystki1, Jeffery L Twiss2

  • 1Department of Drug Discovery & Biomedical Sciences, College of Pharmacy, University of South Carolina Columbia, SC 29208, USA.

bioRxiv : the preprint server for biology
|November 28, 2024
PubMed
概括

在哺乳动物细胞中优化40nm遗传编码纳米颗粒 (GEM) 的表达,提高了细胞质扩散度的测量. 这种改进的跟踪揭示了对细胞平衡和粒子运动动态的洞察力.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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