对PeptideAtlas数据库的深入审计揭示了未注释的编码基因和异常翻译的证据
Jose Manuel Rodriguez1,2, Miguel Maquedano3, Daniel Cerdan-Velez3
1Cardiovascular Proteomics Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 28029 Madrid, Spain.
bioRxiv : the preprint server for biology
|November 28, 2024
概括
研究人员分析了蛋白质组学数据,以找到新的人类蛋白质编码基因和替代蛋白质形式. 他们确定了34个新的未注释的开放阅读框架 (ORF),其中许多可能来自异常翻译.
科学领域:
- 基因组学和蛋白质组学
- 生物信息学是一种生物信息学.
- 分子生物学分子生物学
背景情况:
- 人类基因组已经经历了20多年的广泛研究.
- 新型编码基因经常通过大规模RNA测序,核糖体分析和蛋白质组学实验来确定.
- 参考基因组,如GENCODE,根据实验证据不断更新.
研究的目的:
- 对一个全面的人类蛋白质组学数据库 (PeptideAtlas) 进行深入分析.
- 为了确定编码区域和新型开放阅读框架 (ORF),目前在 GENCODE 参考基因组中没有注释.
- 调查新发现的ORF的起源和保护,并评估异常转换的证据.
主要方法:
- 在人类酸盐 Atlas 数据库中分析蛋白质,和光谱.
- 与GENCODE基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因
- 评估跨物种的ORF保护及其在特定细胞系 (例如癌细胞系) 中的流行情况.
主要成果:
- 识别了数百种可能缺失的替代蛋白质异型和未注释的上游翻译.
- 发现了34个新的未注释的开放阅读框架 (ORF) 的可靠证据.
- 发现近一半的新型ORF属于当前参考集合中缺少的编码基因,而其他则显示出有限的保护性,主要在癌细胞系中检测到,这表明异常翻译.
结论:
- 这项研究提供了强有力的证据,证明了人类基因组中存在众多未注释的编码区域和替代蛋白质异型.
- 很大一部分新型ORF似乎是人类特有的,或者是由于异常转化而产生的,特别是在癌症环境中.
- 这些发现需要重新评估如何将未注释的ORF和潜在的异常翻译纳入参考基因组.
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