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Updated: Jun 6, 2025

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

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通过FDR控制标识传播来改善差异丰富的蛋白质的检测

Alexander J Solivais, Hannah Boekweg, Lloyd M Smith

    bioRxiv : the preprint server for biology
    |November 28, 2024
    PubMed
    概括

    一种新的方法,PIP-ECHO,准确地控制了蛋白质组学中的标识传播 (PIP) 的错误发现率 (FDR). 这提高了差异表达分析的准确性和灵敏性,特别是在单细胞蛋白质组学中.

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    Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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