基转移酶1驱动一个依赖于线粒体的程序,诱导细胞死亡.
Akhilesh Kumar1,2, Corin R O'Shea3, Vikas K Yadav2
1Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, USA.
bioRxiv : the preprint server for biology
|November 28, 2024
概括
基转移酶1 (Ate1) 酶通过在氧化应激下转移到线粒体来调节细胞死亡. 这种线粒体局部化对亡至关重要,独立于细胞质蛋白质降解途径.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 细胞死亡调节对于应激适应和信号传递至关重要.
- 阿尔金转移酶1 (Ate1) 是一种进化保守的酶,参与真核细胞细胞死亡.
- 以前的研究集中在Ate1在蛋白质降解中的细胞质作用上,忽视了它在细胞死亡中的作用.
研究的目的:
- 为了研究 arginyltransferase1 (Ate1) 在细胞死亡中的作用.
- 确定在氧化应激下Ate1的亚细胞定位和功能.
- 为了阐明Ate1诱导的细胞死亡的机制.
主要方法:
- 用了开花酵母作为模型生物.
- 在氧化应激条件下研究了Ate1局部化.
- 分析了Ate1诱导的细胞死亡途径,包括线粒体参与和细胞质降解途径.
主要成果:
- 线粒体转移Ate1是由氧化应激因素诱导的,对于亡至关重要.
- Ate1诱导的细胞死亡取决于线粒体透性孔形成和线粒体因素,但不是直接取决于ROS或ETC活动.
- 细胞质蛋白降解途径 (ubiquitin-proteasome,自,ER压力) 并没有显著影响Ate1诱导的细胞死亡.
结论:
- 基转移酶1 (Ate1) 控制了一种依赖于线粒体的细胞死亡途径.
- 线粒体中Ate1的定位是压力诱导的亡的一个关键事件.
- Ate1在细胞死亡中的作用主要是线粒体的,与其已知的细胞分裂功能不同.
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