在肠道微生物群中通过产生多样性的反元素来向蛋白质进化
Benjamin R Macadangdang1,2, Yanling Wang3, Cora Woodward2
1Division of Neonatology and Developmental Biology, Department of Pediatrics, David Geffen School of Medicine at the University of California, Los Angeles, Los Angeles, CA, United States.
bioRxiv : the preprint server for biology
|November 28, 2024
概括
肠道微生物群中的多样性生成反元素 (DGRs) 快速演变为细菌蛋白质. 这些DGR在物种之间转移,影响宿主微生物相互作用和婴儿肠道殖民.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 基因组学就是基因组学.
背景情况:
- 众所周知,产生多样性的逆元件 (DGR) 可以加速蛋白质进化.
- 人的肠道微生物群具有高密度的DGRs,表明关键的适应性作用.
- 细菌类物种是人类肠道微生物群的关键成员.
研究的目的:
- 研究DGRs在与人类相关的Bacteroides物种中的多样性,活性和功能.
- 了解DGR水平转移的机制及其对蛋白质多样化的影响.
- 探索DGRs在宿主微生物相互作用和婴儿肠道殖民中的作用.
主要方法:
- 在Bacteroides物种中,生物信息识别和分析了超过1100个独特的DGR.
- 对DGR活动和水平转移的实验性表征.
- 在gnotobiotic小鼠中进行竞争测试,以评估DGR多样化的蛋白质的适应意义.
- 在母婴配对中,分析了多个不同类型的2,700多个DGR.
主要成果:
- 细菌体DGR的一个子集使V型 pili和相关蛋白质的粘合成分多样化.
- 细菌类DGR在物种之间水平转移,活动水平各不相同.
- DGRs优先改变粘合器官上的联结残留物的功能特征.
- 特定的DGR驱动的蛋白质变体在与其他共生细菌的竞争中得到丰富.
- 细菌类DGRs优先转移到阴道分娩的婴儿,并积极多样化.
结论:
- 细菌体DGR在人类肠道的适应性进化中发挥着重要作用.
- DGRs的水平转移和有针对性的多样化有助于塑造与宿主相关的微生物群落.
- DGRs代表了微生物进化中随机,向基因组可塑性的关键机制.
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