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Updated: Jun 6, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

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跨平台的DNA基因发现和基准测试,以探索研究不足的人类转录因子的结合特异性

Ilya E Vorontsov1,2, Ivan Kozin3, Sergey Abramov1,4

  • 1Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia.

bioRxiv : the preprint server for biology
|November 28, 2024
PubMed
概括

这项研究使用大规模数据评估了DNA模式发现工具,发现大多数工具在高质量的实验数据中表现良好. 一个新的DNA图案在线目录被创建,以帮助未来的研究.

关键词:
这就是ChIP-Seq.具有DNA结合的特异性发现DNA动机的发现在GHT-SELEX中.在HT-SELEXEX中使用.这就是PBM的PBM.在PSSM中,PSSM可以使用PSSM.在PWM中使用PWM.在SmiLE-Seqq中使用.根据TFBS的规定,TFBS可以基准测试 (benchmarking) 是一种比较的方法.位置重量矩阵的位置重量矩阵.转录因子的结合点是转录因子的结合点.转录因子是一种转录因子.

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相关概念视频

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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