两个入侵性Adelges物种的基因组 (hemlock woolly adelgid和胆 adelgid) 允许对尼古丁酸乙胆受体进行表征
A M Glendening1, Cole Stephens2, Vidya S Vuruputoor1
1Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, USA 06269.
bioRxiv : the preprint server for biology
|November 28, 2024
概括
两种入侵性 adelgid 物种 Adelges tsugae 和 A. abietis 的基因组测序,提供了对它们的生物学和开发有针对性的害虫控制策略的潜力的见解. 这项研究有助于保护北美针叶树免受这些有害昆虫的侵害.
科学领域:
- 基因组学和生物信息学
- 昆虫学 昆虫学是一门学科.
- 林业科学 林业科学
背景情况:
- 两个入侵性的半角类 adelgids,Adelges tsugae (hemlock woolly adelgid) 和A. abietis,对北美针叶树造成重大破坏.
- 目前的广泛杀虫剂治疗对有益的昆虫产生了非目标影响,需要开发更有选择性的控制方法.
- 了解这些入侵物种的遗传构成对于制定有针对性的害虫控制和保护策略至关重要.
研究的目的:
- 执行Adelges tsugae和A. abietis的全基因组测序,以确定选择性害虫控制的目标.
- 分析基因家族,并确定与这些 adelgids 的入侵性质和宿主相互作用相关的扩张或收缩.
- 研究尼古丁酸乙胆受体 (nAChRs) 作为新型杀虫剂的潜在点的保护.
主要方法:
- 全基因组测序和A. tsugae (220.75 Mbp) 和A. abietis (253.16 Mbp) 的组装.
- 基因组注释以识别编码蛋白质的基因 (11,424 在A. tsugae, 14,118 在A. abietis).
- 对29种半类动物和14种关节动物外群进行比较基因组分析,包括对nAChR子单元的基因组分析.
主要成果:
- 这两种基因组都超过96%的完整性,每个基因组都包含9个染色体.
- 在A. abietis中的基因家族扩展包括ABC载体和碳氧酶;这两种物种都显示了基因素和氧化还原酶通路的收缩.
- A. tsugae显示了与细胞分化和植物免疫抑制相关的基因的扩张,而nAChR显示了跨物种的保存特征.
结论:
- 组装的基因组为了解 adelgid 生物学和开发有针对性的控制策略提供了基础.
- 扩展基因家族的识别为新的害虫管理方法提供了潜在的目标.
- 甲类动物中nAChRs的保存性表明它们可以作为开发选择性杀虫剂以保护针叶树林的目标.
关键词:
在Cys带通道离子通道受体 (Cys-LGIC) 中.它们是 adelgid 类的.针叶树是一种树.保护 保护 保护森林树的健康 森林树的健康杀虫剂是一种杀虫剂.侵入性物种是一种入侵性物种.参考基因组是指基因组中的基因组.更多相关视频
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