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单细胞表观遗传分析揭示了与癌中BAP1缺乏相关的干扰素反应高的程序

Sabrina Y Camp^1,2, Meng Xiao He^1,2,3, Michael S Cuoco^2,4

¹Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.

bioRxiv : the preprint server for biology

|November 28, 2024

概括

细胞癌 (RCC) 的表观遗传变化揭示了四个恶性程序. BAP1突变与干扰素反应和清晰细胞RCC的不良预后有关,可能通过ERV3-16A3_LTR.

科学领域:

基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
在瘤学瘤学.

背景情况:

细胞癌 (RCC) 具有重复的表观遗传突变,定义了患者亚组.
细胞类型特异性RCC的表观遗传特征,特别是关于突变的表观遗传特征,尚未完全理解.

研究的目的:

为了研究RCC在不同突变中的表观遗传情景.
在透明细胞RCC (ccRCC) 中识别细胞类型特定的表观遗传程序.

主要方法:

整合来自四个独立RCC队列的单核ATAC测序数据.
与突变相关的染色质可访问性和基因表达的分析.

主要成果:

在ccRCC中发现的四个共享的恶性表观遗传程序:血管新生,近端管状特征,干扰素 (IFN) 信号传递,以及缺乏明显可访问区域的程序.
BAP1突变显著影响了染色质的可访问性,与IFN反应联系在一起.
增加的IFN信号来源包括免疫透和增加ERV3-16A3_LTR可访问性/表达.
在ccRCC中,ERV3-16A3_LTR表达可能是一个负的预后生物标志物.

结论:

恶性表观遗传程序汇聚在ccRCC瘤中.
BAP1损失与IFN响应高的表观遗传程序有关,可能由ERV3-16A3_LTR失调调节介导.

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