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通过液体激发来聚合Transthyretin

Irina Ritsch¹, H Jane Dyson¹, Peter E Wright¹

¹Department of Integrative Structural and Computational Biology and Skaggs Institute of Chemical Biology, Scripps Research, 10550 North Torrey Pines Road, La Jolla, CA 92037.

bioRxiv : the preprint server for biology

|November 28, 2024

概括

剪切力,就像血液流中的剪切力一样,可以触发TTR聚合,导致粉样化症. 这项研究揭示了TTR四相解离和中间状态如何在生理条件下促进聚合.

科学领域:

生物化学生物化学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

晶氨酸 (TTR) 携带甲状腺素和视网醇结合蛋白.
TTR聚合会导致与神经退行性疾病和心脏病相关的跨甲基氨基粉症 (ATTR).
在生理条件下TTR聚合的触发因素仍然未知.

研究的目的:

在中性pH下调查导致TTR聚合的因素.
阐明剪切力在TTR聚合中的作用.

主要方法:

通过动TTR溶液来产生流体剪切力.
使用NMR光谱学监测TTR聚合并分析结构变化.

主要成果:

激发TTR溶液诱导的富含β聚合物和纤维.
聚合率取决于蛋白质度,在较高度下延迟时间较长.
核磁共振揭示了一种动态乱的四聚体中间体,表明了不稳定的二聚体-二聚体接口.

结论:

流体剪切力可以在中性pH时启动TTR聚合.
聚合涉及四聚体解离,单聚体展开,以及聚合倾向的中间体的形成.
了解这些机制,可以了解ATTR的病原性.

关键词:

这就是NMR的NMR.光是一种光.西胺氨基化剂 transthyretin 是一种

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