乙醇胺诱导的微分区组合对于菌核酸菌的毒性是必要的
Dana S Franklin1, Yi-Wei Chen2, Yimin Chen2
1Molecular Biology Institute, University of California, Los Angeles, California, USA.
bioRxiv : the preprint server for biology
|November 28, 2024
概括
细菌微分区 (BMCs) 在Fusobacterium nucleatum中调解乙醇胺代谢. 这一由两部分组成的系统调节的过程对于细菌生长和毒性至关重要,包括在小鼠模型中诱导早产.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌病原体的产生
背景情况:
- 细菌通过细菌微分区 (BMC) 使用乙醇胺作为营养物质.
- 一种口腔病原体Fusobacterium nucleatum与不良妊娠结果有关.
- 核菌的基因组含有乙醇胺利用 (eut) 位点,其中含有BMC和代谢的基因.
研究的目的:
- 研究F.核中BMCs的组装,调节和功能.
- 确定BMCs在乙醇胺代谢和细菌毒性中的作用.
- 阐明BMC形成的遗传调节.
主要方法:
- 对 eut locus 的基因表达分析.
- 用乙醇胺和胎盘细胞培养细菌.
- 基因删除突变的构建和分析 (eutV,eutN,eutL/eutM).
- 质谱测量用于BMC蛋白质识别.
- 过早分娩的小鼠模型.
主要成果:
- 乙醇胺显著上调F. nucleatum.中的eut基因表达和BMC形成.
- 两个组成的系统EutV-EutW在对乙醇胺的反应中通过转录来调节BMC的形成.
- BMC外蛋白 (EutL,EutM1,EutM2,EutN) 对于BMC组装和乙醇胺利用至关重要.
- 缺乏关键BMC蛋白质的突变者表现出生长缺陷,并且在小鼠模型中无法诱导早产.
结论:
- BMC介导的乙醇胺代谢对于F. nucleatum的生长和毒性至关重要.
- 欧特维-欧特维TCS控制BMC的形成,以响应营养的可用性.
- BMC的组装和功能对于F. nucleatum在不良妊娠结果,特别是早产中所起的作用至关重要.
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