累积细胞DNA损伤与排卵功能障碍表型的女性受精成功有关

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Updated: Jun 6, 2025

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

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Published on: June 23, 2023

Bárbara Rodrigues^1,2,3, Vanessa Sousa^1,2,3, Filipa Esteves^3,4,5

¹Molecular Genetics Laboratory, Laboratory Genetics Service, Genetics and Pathology Clinic, Centro Hospitalar Universitário de Santo António, Unidade Local de Saúde de Santo António, Porto, Portugal.

Frontiers in cell and developmental biology

|November 28, 2024

概括

围绕卵子的累积细胞中的DNA损伤可能预测细胞内质精子注射 (ICSI) 周期中的受精成功. 这些细胞中较高的DNA损伤与排卵功能障碍的女性更好的受精结果相关.

科学领域:

生殖生物学生殖生物学
细胞和分子生物学是细胞和分子生物学.
不孕症的研究研究.

背景情况:

内细胞质精子注射 (ICSI) 是一种关键的生育治疗方法.
卵细胞的成熟受周围的累积细胞的影响,对ICSI成功至关重要.
累积细胞中DNA损伤作为受精预测者的作用还未得到充分研究.

研究的目的:

调查在ICSI过程中累积细胞中的DNA损伤与卵细胞能力之间的联系.
评估累积细胞中的DNA损伤是否可以预测体外受精 (IVF) 的结果.
为了比较累积细胞和外围血液中的DNA损伤水平.

主要方法:

性彗星试验被用来测量DNA损伤 (%TDNA).
样本包括从接受ICSI的潜在生育和不育女性的累积细胞和血液.
特别关注的是患有排卵功能障碍的不孕女性.

主要成果:

在累积细胞和血液之间发现了%TDNA的显著差异.
患有排卵功能障碍的女性在累积细胞中显示TDNA的%比肥沃对照更高.
在排卵功能障碍的病例中,较高的累积细胞%TDNA与更成功受精的卵细胞 (2-pronuclei) 相相关.

结论:

血液DNA损伤并不反映累积细胞DNA损伤.
累积细胞DNA损伤是ICSI中受精成功的潜在生物标志物.
累积细胞中DNA损伤的增加可能表明更好的卵细胞能力和受精可能性.

关键词:

造成的DNA损伤是DNA损伤.彗星测试试验彗星测试试验累积细胞是累积的细胞.授精成功成功成功的受精排卵功能障碍排卵功能障碍有两个前核.

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