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Updated: Jun 6, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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Published on: April 30, 2018

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用DNA引导的CRISPR/Cas12用于RNA向

Carlos Orosco1, Santosh R Rananaware1, Boyu Huang1

  • 1Department of Chemical Engineering, University of Florida, Gainesville, FL, USA.

medRxiv : the preprint server for health sciences
|November 28, 2024
PubMed
概括

研究人员开发了 ΨDNA,这是一种基于 DNA 的 Cas12 酶指南,可用于诊断和细胞 RNA 调节的精确 RNA 向. 这项创新绕过了传统的RNA指南,用于增强CRISPR应用.

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CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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