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在患有食道状细胞癌的患者中,组织来源可能会影响食道菌群
Xiao-Bo Liu1,2,3, Zi-Ye Gao4, Wen Xu2
1Department of Gastroenterology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
Oncology letters
|November 28, 2024
概括
这项研究比较了食道状细胞癌 (ESCC) 患者的手术和内镜组织中的食道微生物群. 手术后的组织显示出更高的细菌多样性,这表明它们更适合ESCC微生物群研究.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 食道状细胞癌 (ESCC) 研究越来越多地关注食道微生物群的作用.
- 标准化采样方法对于可靠的ESCC微生物群研究至关重要.
- 外科手术和内镜组织样本之间的微生物群组成的差异还没有得到很好的定义.
研究的目的:
- 为了比较ESCC患者手术 (术后) 和内镜食道粘膜组织之间的细菌群体差异.
- 为在ESCC微生物群研究中选择最佳采样方法提供理论基础.
- 评估这些组织在未来微生物群研究中的潜力.
主要方法:
- 收集了72名ESCC患者的食道组织样本.
- 扩大了16S rRNA基因的V4超变区.
- 进行了Illumina HiSeq测序并分析了α和β多样性.
- 利用线性差异分析效果大小 (LEfSe) 和随机森林建模.
主要成果:
- 与粘膜组织 (B组) 相比,术后组织 (A组) 的Shannon和Chao1多样性指数显著更高.
- 根据LEfSe的分析,细菌的丰度明显,A组的Megasphaera,Actinobacteria,Enterobacteriaceae和Enterobacteriales含量更高,而B组的Mogibacteriaceae含量更高.
- 一个随机森林模型准确地区分了样本组 (AUC=0.86),表明可靠的分类.
结论:
- 样本来源显著影响ESCC患者的食道微生物群概况.
- 手术后的食道组织表现出更大的微生物丰富性和均性,使它们更适合ESCC微生物群研究.
- 考虑样本来源对于食道微生物群研究中的一致和可重现的发现至关重要.
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