与长间隔元素-1编码ORF1p相关的多个RNA结合蛋白被系统性红斑狼的自身免疫反应所准
Kennedy C Ukadike1,2, Alyssa N Colyer1, Bhargavi Duvvuri1
1Division of Rheumatology, Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA.
概括
系统性红斑狼 (SLE) 患者对ORF1p和RNA颗粒中的其他八种蛋白质具有IgG自身抗体. 这些自身抗体与疾病活性和I型干扰素水平相关.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 类风湿病学 类风湿病学
背景情况:
- 系统性红斑狼 (SLE) 是一种自身免疫性疾病,其特征是对核酸和相关蛋白质的自身抗体.
- 来自长间隔元素-1 (LINE-1) 的ORF1p蛋白是SLE中已知的自身抗原.
- 在宏分子组合中,ORF1p与RO60和其他RNA结合蛋白相结合.
研究的目的:
- 调查是否与ORF1p共定位的其他蛋白质也是SLE患者IgG自身抗体的标.
- 确定这些自身抗体与SLE的临床特征和疾病活性之间的相关性.
主要方法:
- 与酶相关的免疫吸收试验 (ELISA) 和免疫血块用于检测SLE患者血清中的IgG自身抗体.
- 对ORF1p和其他八种候选蛋白质 (LARP7,MOV10,ZCCHC3,MEPCE,YARS2,RPL18A,RPL27A,H2BC17) 的抗体反应性进行了评估.
- 进行了集群分析,以将自身抗体配置文件与疾病活性和临床表现相关联.
主要成果:
- 与健康对照组不同的是,SLE患者对LARP7,MOV10,ZCCHC3,MEPCE,YARS2,RPL18A,RPL27A和H2BC17的IgG自身抗体增加.
- 对RO60,LARP7,MOV10和MEPCE的抗体标位在具有抗ORF1p自身抗体的患者中较高.
- 这些自身抗体与抗dsDNA抗体,关节炎,I型干扰素升高以及整体疾病活性相关.
结论:
- 患有SLE的患者对ORF1p和RNA丰富颗粒中发现的其他八种蛋白质具有较高的IgG自身抗体.
- 这些自身抗体与更高的疾病活性有关,特别是在具有抗ORF1p抗体的患者中.
- 含有ORF1p的核糖蛋白颗粒是系统性红斑狼自身免疫的潜在目标.
关键词:
关节炎是一种关节炎.自动抗体是一种自身抗体.线路-1 线路-1 在线MOV1010 在线视频播放在ORF1p中,ORF1p是在Ro6060上,我们可以看到RO60的位置.系统性红血性狼 (Systemic lupus erythematosusus) 是一种全身性狼.这是一种I型干扰子.更多相关视频
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