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复习Trypanosoma rangeli在脊椎动物宿主中的发展

Luan Felipe Santos¹, Flávia de Souza Rocha², Marcelo Gustavo Lorenzo^1,3

¹Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Grupo de Comportamento de Vetores e Interação com Patógenos, Belo Horizonte, MG, Brasil.

Memorias do Instituto Oswaldo Cruz

|November 28, 2024

概括

在老鼠中,Trypanosoma rangeli会产生持续的感染,在从血液中消失很久后,在淋巴体器官中检测到,导致生理变化. 需要进一步研究其对哺乳动物宿主的影响.

科学领域:

寄生虫学的寄生虫学
哺乳动物感染模型
分子诊断学分子诊断学

背景情况:

兰基虫 (Trypanosoma rangeli) 是一种在南美洲和中美洲发现的血虫寄生虫.
它与Trypanosoma cruzi共同存在,这是查加斯病的原因,导致混合感染和诊断挑战.
哺乳动物中T. rangeli的繁殖形式仍然没有被描述.

研究的目的:

为了调查Trypanosoma rangeli感染在哺乳动物宿主中的发展和持久性.
在长时间内评估关键的感染参数.
了解T. rangeli在哺乳动物组织中的长期存在.

主要方法:

瑞士韦伯斯特小鼠是通过受感染的Rhodnius prolixus的咬伤感染的.
寄生病,体温和体重被监测了120天.
在第132天使用PCR和qPCR检测到T. rangeliDNA在脏和介质淋巴结中.

主要成果:

从第二天开始,T. rangeli在血液中被检测到,在第五天达到峰值,并在第120天变得无法检测到.
在感染后的淋巴体器官 (脏和淋巴结) 中确认了T. rangeli DNA.
与对照组相比,受感染的小鼠体温升高,体重增加减少.

结论:

在老鼠中,Trypanosoma rangeli建立了持续的感染,在血液清理后很长一段时间,在淋巴体器官中检测到DNA.
受感染的小鼠表现出生理变化,表明潜在的亚临床影响.
需要进一步研究宿主免疫反应和T. rangeli对哺乳动物健康的影响.

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