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来自Polygonum cuspidatum Siebold & Zucc的Resveratrol的可持续和综合微生物生物催化剂,使用基于纤维素的不移的黑色阿斯伯吉洛斯与深度欧特克溶剂辅助微反应器

Shuang Jin¹, Yubin Ren², Cailiang Peng³

¹College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, 150040, People's Republic of China. jinshuangzy@126.com.

Applied biochemistry and biotechnology

|November 28, 2024

概括

绿色的深层欧特溶剂 (DES) 增强了 Polygonum cuspidatum 使用 Aspergillus niger 的复星产量. 自然DES (NADES) 提高了产量13倍,显示出高生物催化剂稳定性和环保潜力.

科学领域:

生物技术是生物技术.
绿色化学绿色化学
酶催化酶的催化作用

背景情况:

Resveratrol是一种有价值的stilbenoid,主要是从Polygonum cuspidatum中提取出来的.
传统的提取方法通常涉及苛刻的化学品和低产量.
生物催化提供了一种可持续的替代方案来生产白醇.

研究的目的:

开发一种高效,环保的白醇生物转化方法.
为了研究使用深度环氧溶剂 (DES) 来增强生物催化剂的使用.
为了优化条件,最大限度地提高resveratrol产量,使用固定Aspergillus niger.

主要方法:

选各种DES和天然的深溶性溶剂 (NADES) 用于生物催化增强.
使用基于纤维素的动不动的黑色阿斯伯吉洛斯来生产白醇.
优化反应参数,包括pH值,温度,溶剂度和时间.

主要成果:

与传统的DES相比,NADES显示出更高的生物相容性和催化作用.
最佳条件 (pH6.5,29.5°C,1.0重%CHCL/EG) 产生了32.79 mg/g的白醇,增加了13.06倍.
固定生物催化剂在10个周期后保持了81.46%的活性,表明其稳定性很好.

结论:

深度欧性溶剂辅助生物转化是一种高效和绿色的方法,用于产生白醇.
对于生物催化剂,NADES在生物相容性和催化效率方面具有显著的优势.
开发的方法为传统的白醇提取技术提供了一个可持续和可扩展的替代方案.

关键词:

多边形的顶端 (Polygonum cuspidatum) 是由西博尔德和扎克斯创立的.生物转化生物转化基于纤维素的微反应器深度欧性溶剂是一种溶剂.复星醇是一种复星.

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