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功能性特征,转录组和代谢组分析显示,pacR在Xanthomonas campestris pathovar campestris中具有多方面的生理作用

Chao-Tsai Liao¹, Hsiao-Ching Chang¹, Chih-En Li¹

¹Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan.

Microbial pathogenesis

|November 28, 2024

概括

在Xanthomonas campestris pv.中pacR基因的突变坎佩斯特里斯减少了细菌的附着,毒性,并改变了基因和代谢物概况. 这项研究揭示了PacR.

科学领域:

植物病理学植物病理学
细菌遗传学细菌遗传学
分子微生物学分子微生物学

背景情况:

桑托蒙纳斯·坎佩斯特里斯 (Xanthomonas campestris) 和帕索瓦尔·坎佩斯特里斯 (Pathovar campestris) (Xcc) 是导致十字树的黑色腐烂的原因.
假设蛋白质的功能,比如由AAW18_RS04175 (pacR) 编码的蛋白质,往往是不太了解的.

研究的目的:

为了研究在Xcc.中假设蛋白PacR (DUF1631) 的功能.
阐明 pacR 突变对 Xcc 生理学,毒性和基因/代谢物表达的影响.

主要方法:

在Xcc菌株Xc17中产生一个pacR突变.
评估细菌的附着,外聚糖的产生,过敏反应和毒性.
进行转录组和代谢组分析.
使用定量RT-PCR和促进体分析.

主要成果:

帕克R突变降低了细菌的附着,外聚糖的产生,HR和毒性.
突变者显示细胞膜完整性降低和外部膜囊泡的产生.
转录组分析显示了225个基因的差异表达,包括那些参与T3SS和核酸代谢的基因.
代谢分析发现了81个和132个代谢物的显著变化 (分别是正和负模式).

结论:

在Xcc附着,外聚糖生成,膜完整性和毒性方面,PacR起着至关重要的作用.
PacR影响了与分泌系统和新陈代谢相关的基因的表达.
这项研究为含有DUF1631的蛋白质在细菌生理学和病变发生过程中的功能提供了新的见解.

关键词:

细菌的附着物是细菌的附着物.致病性致病性致病性克桑托蒙纳斯 (Xanthomonas) 是一种

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