来自人类,动物和环境的大肠杆菌中的抗菌素耐药性基因和相关的移动遗传元素
Chanchal Rana1, Vaibhav Vikas2, Saraswati Awasthi3
1ICAR-National Dairy Research Institute (NDRI), Animal Biotechnology Division, Animal Genomics Lab, Karnal, 132001, Haryana, India.
Chemosphere
|November 28, 2024
概括
抗微生物耐药性基因 (ARG) 通过像大肠杆菌这样的细菌中的移动遗传元素在全球范围内传播. 了解这些遗传框架,特别是鸟类中与IS相关的ARG,是打击抗菌素耐药性 (AMR) 的关键.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 公共卫生 公共卫生
背景情况:
- 全球抗菌素耐药性 (AMR) 的上升是一个重大威胁.
- 环境有助于人类和动物之间传播抗微生物耐药性基因 (ARG).
- 细菌群体在移动遗传元素 (MGE) 上承载着多样化的ARG,例如等离子体和插入序列 (IS).
研究的目的:
- 调查ARG患病率及其遗传框架.
- 从全球多种来源分析大肠杆菌 (E. coli) 的整个基因组序列.
- 评估遗传背景对ARG传播潜力的影响.
主要方法:
- 从全球的人类,动物,鸟类和环境来源获取和分析了2199个大肠杆菌全基因组序列.
- 研究了ARG的流行率及其与移动遗传元素 (MGE) 的关联,包括质粒,IS元素和转子.
- 确定了特定的IS-ARG关联和传播的携带ARG的MGEs.
主要成果:
- 在英国和美国发现的最大ARG,主要在人类宿主中.
- 与IS相关的ARG在鸟类宿主中最为普遍.
- 在所有主机中,广泛的ARG包括aph6ld,aph3lb,blaCTX-M和blaNDM. Tn2是最常见的MGE,通常在IncFIB等离子体上. IS26和ISVsa3与氨基糖化物和β-乳酸盐耐药性有关. 观察到固定的关联,例如,mph(A) _IS6100和blaNDM-5_IS5,其中blaNDM和blaCTX-M变体与IS元素密切相关.
结论:
- 遗传框架,特别是围绕ARG的MGEs,显著影响它们的传播潜力.
- 鸟类中与IS相关的ARG代表着一个显著的储存和传播途径.
- 了解ARG-MGE近距离提供了预测和打击AMR的策略.
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