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使用便携式设备和纳米孔测序器对18-STR长安普利康面板进行快速测序和识别
Jiarong Zhang1, Tingting Yang1, Zihan Xie2
1School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, PR China; Academy of Military Medical Science, Beijing 100850, PR China; Shanxi Key Laboratory of Forensic Medicine, Jinzhong 030600, PR China.
Genomics
|November 28, 2024
概括
这项研究介绍了一种快速纳米孔测序方法,用于使用长安普利康STR面板进行法医人类识别. 这种方法可以在现场应用中进行准确的识别,克服以前方法的局限性.
科学领域:
- 法医遗传学 法医遗传学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 短串联重复 (STR) 是人类识别的关键法医标记.
- 纳米孔测序为遗传分析提供了可移植性和高数据吞吐量.
- 现有的基于联结的STR分析库准备是耗时的,不适合现场使用.
研究的目的:
- 开发一种快速,可在现场部署的纳米孔测序方法,用于STR分析.
- 在快速图书馆准备方法中克服小安普利康的局限性.
- 评估开发的法医识别方法的准确性和效率.
主要方法:
- 开发了一个18-STR复杂面板,带有长安普利康 (~1.4 Kbp).
- 优化PCR条件,可在2小时12分钟内完成.
- 使用基于转酶的快速库准备方法,与随机碎片化兼容.
- 在24个样本上使用便携式纳米孔测序仪验证了该方法.
主要成果:
- 使用快速库准备,成功地进行了长安普利康 (~1.4 Kbp) 的分析.
- 平均29.16%的读数覆盖了整个STR区域,足以进行分析.
- 整个过程,包括图书馆的准备和排序,花了10.5小时.
- 在对对识别分析中获得了95.36%的准确性.
结论:
- 开发的长安普利康STR面板和快速库准备方法对于基于纳米孔的法医应用是可行的.
- 这种方法可以在现场环境中快速,便携且准确地识别人类.
- 纳米孔测序显示了推进法医科学能力的巨大潜力.
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