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验证crAssphage微生物源追踪标记物和与Bacteroidales标记物进行比较,以检测和量化表面水中的便污染物

Shuang Liu¹, Trillion Surya Lioe², Li Sun³

¹Department of Biosciences and Bioinformatics, School of Science, Xi'an Jiaotong-Liverpool University, Suzhou, 215123, China; Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, L69 7ZX, UK.

Environmental pollution (Barking, Essex : 1987)

|November 28, 2024

概括

新的crAssphage标记器准确地检测到中国地表水中的人类便污染,性能优于旧的方法. 这些工具改善了对环境和公共卫生保护的监测.

科学领域:

环境微生物学环境微生物学
水质监测监测水质监测水质
公共卫生公共卫生

背景情况:

人类特有的便污染威胁到地表水,对生态系统和公共卫生构成风险,因为潜在的病原体共同发生.
微生物源追踪 (MST) 使用遗传标记来识别便污染源,这对于有效的水资源管理至关重要.
传统的MST标记物如Bacteroidales在人类废物检测的特异性和准确性方面存在局限性.

研究的目的:

评估crAssphage标记物 (CPQ_056和CPQ_064) 在检测中国地表水中的家用污水污染时的有效性.
为了比较crAssphage标记物与传统的Bacteroidales标记物的性能,用于微生物源追踪.
评估太湖流域的空间分布和便污染源.

主要方法:

使用污水和动物便样本验证crAssphage标记物 (CPQ_056,CPQ_064) 和传统标记物 (BacUni,HF183 TaqMan,Pig-2-Bac,GFD).
在2020年夏季和冬季从太湖流入河流收集的地表水样本中量化MST标记物.
空间分析和与水质参数和土地使用数据的相关性.

主要成果:

形形标记物CPQ_056和CPQ_064表现出高灵敏度和特异性 (分别为100%/96.7%和100%/100%),表现优于HF183 (100%/80.4%).
在所有样本中,污水/废水被确定为便污染的主要来源.
在西北河流中观察到较高的污染水平,与人类活动和特定的土地用途相关,并注意到下游的稀释模式.

结论:

CrAssphage标记器为监测地表水中的人类便污染提供了更高的精度和准确性.
这些先进的MST工具为决策者和环境管理者提供了有价值的数据,以解决水污染问题.
这项研究强调了crAssphage在识别家用污水作为泰湖流域污染的主要来源方面的有效性.

关键词:

这种细菌是 Bacteroidales.便污染便污染淡水污染淡水污染遗传标记物遗传标记物微生物源跟踪追踪微生物源跟踪在Crassphage中使用.

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