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通过NF-κB信号通路将MIR-15a-5p的Knockdown调高ABCB1表达和降低HNSCC进展

Jing Liu¹, Chaoyang Lv², Sis Aghayants³

¹Outpatient Department, Renmin Hospital of Wuhan University, Wuhan, China.

Journal of investigative surgery : the official journal of the Academy of Surgical Research

|November 28, 2024

概括

在头部和部状细胞癌 (HNSCC) 中,抑制miR-15a-5p会增加ABCB1水平,通过NF-κB途径抑制瘤的进展. 这些发现确定了ABCB1和miR-15a-5p作为潜在的HNSCC生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

头部和部状细胞癌 (HNSCC) 的特点是高侵袭性和异质性,导致临床结果差.
可靠的生物标志物对于改善HNSCC诊断和治疗策略至关重要.

研究的目的:

为了确定头部和部状细胞癌 (HNSCC) 的新型分子生物标志物.
研究微RNA (miRNA) 和它们的基因在HNSCC进展中的调控作用.

主要方法:

使用cBioPortal数据库对经常突变的基因进行查.
进行miRNA-mRNA联合表达分析以识别相互作用的miRNAs.
在体外测试 (CCK-8,Transwell) 来评估细胞增殖,迁移和侵入.
使用异种移植小鼠模型进行体内研究,以评估治疗疗效.

主要成果:

ATP结合盒载体1 (ABCB1) 在HNSCC中表现出最高的突变频率;降低的ABCB1与更好的患者预后相关.
确定了米R-15a-5p作为ABCB1.1的调节者. 抑制miR-15a-5p导致ABCB1上调.
抑制miR-15a-5p在体外显著抑制了HNSCC细胞的增殖,迁移和入侵,并在体内减少瘤生长和转移,可能是通过NF-κB信号通路.

结论:

消除MiR-15a-5p可以增强ABCB1的表达,从而通过NF-κB信号通路抑制HNSCC的进展.
在HNSCC中,ABCB1和miR-15a-5p显示出作为治疗干预的预测生物标志物的潜力.

关键词:

在ABCB1中,ABCB1是ABCB1.在HNSCC中,我们可以看到HNSCC.在 miR-15a-5p 中.预后预后预后这是一个渐进的过程.

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