相关实验视频

Updated: Jun 6, 2025

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

一个基于世卫组织2021年的综合方案,概述了精子参数与PGT-A周期中IVF结果的关联

Rossella Mazzilli^1,2, Danilo Cimadomo², Federica Innocenti²

¹Department of Clinical and Molecular Medicine, University "Sapienza" of Rome, Rome, Italy.

|November 28, 2024

概括

精液质量差,包括精子度,形态和运动率低,在ICSI周期中显著降低了euploidy胚胎细胞率和活产机会. 优化精子参数对于男性因子不孕症治疗成功至关重要.

科学领域:

生殖医学生殖医学
遗传学是一个遗传学.
安德罗学与人类学

背景情况:

精液参数和父亲因素,如BMI和年龄在辅助生殖技术中至关重要.
世界卫生组织 (WHO) -2021年标准为精液分析提供了最新的指南.

研究的目的:

调查精液参数 (WHO-2021),父亲的BMI和年龄与胚胎和临床结果之间的关联.
评估这些因素对细胞内质精子注射 (ICSI) 循环的影响,并进行植入前遗传测试以检测形积分 (PGT-A).

主要方法:

从3101对 (2013-2021) 的4013个ICSI + PGT-A周期进行了回顾性分析.
进行了皮活检和综合染色体检测.
调整为混因素的回归分析评估了精液特征,父亲的BMI/年龄和结果之间的关联.

主要成果:

孕产妇的年龄是euploidy胚胎细胞发育率 (EBR) 的主要混因素.
精液参数低于第五百分位数 (运动性,度,形态) 和亚精 (OA,NOA) 与明显较低的EBR相关.
低精子度,形态和运动性,以及OA,在调整为母亲的年龄和卵细胞数量后,显著降低了活产的机会.

结论:

精液质量显著影响ICSI-PGT-A结果,影响euploidy率和活产成功.
父亲年龄超过44岁与胚胎细胞质量降低有关.
临床医生应该就这些因素向夫妇提供建议,并考虑改善男性因子不孕症的精子参数.

关键词:

美国国务院调查局 (ICSI)在PGT-A中.累积活产率是指累积的活产率.父亲的年龄父亲年龄精子因子精子因子

更多相关视频

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

相关概念视频

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

Infertility in Males

Infertility in Males

Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...