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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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相关实验视频

Updated: Jun 23, 2026

Denver Papillae Protocol for Objective Analysis of Fungiform Papillae
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与版本 1 相比,PI-QUAL 2 版本的图像质量分类和读者间协议与版本 1 相比.

Kang-Lung Lee1,2,3, Iztok Caglic4, Po-Hsiang Liao3,5,6

  • 1Department of Radiology, University of Cambridge, Cambridge, UK.

European radiology
|November 28, 2024
PubMed
概括
此摘要是机器生成的。

使用PI-QUAL v1和v2进行前列腺MRI质量评估的读者间协议是可比的. 版本2显示了从最佳到可接受质量的转变,动态对比度增强提高了整体MRI质量.

关键词:
这就是为什么MRI是MRI.这就是PI-QUAL.前列腺前列腺前列腺质量质量质量质量质量质量质量质量.

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科学领域:

  • 放射学 放射学是一门学科.
  • 医疗成像医学成像
  • 质量评估的质量评估

背景情况:

  • 前列腺成像质量 (PI-QUAL) 标准化前列腺MRI评估.
  • PI-QUAL已更新到版本2,需要对其性能进行评估.

研究的目的:

  • 评估PI-QUAL v1和v2的读者之间的协议.
  • 为了研究PI-QUAL版本之间的MRI质量评分类别的变化.

主要方法:

  • 350个多参数MRI扫描 (mpMRI) 的回顾性分析.
  • 两位泌尿放射学家使用PI-QUAL v1和v2.2进行mpMRI质量的独立评估.
  • 使用kappa统计数据确定读者之间的协议;通过奇平方测试进行分类比较.

主要成果:

  • 对PI-QUAL v1和v2 (卡帕值在0.54到0.64之间) 观察到实质性至中度的读者间一致.
  • 质量水平从以v1为主的"最佳"转向以v2为主的"可接受" (p < 0.001).
  • 动态对比增强 (DCE) 提高了质量,从双参数MRI (bpMRI) 中的"不足"到mpMRI 中的"可接受" (p < 0.001).

结论:

  • PI-QUAL v1 和 v2 显示了整体和个体序列分类的一致的读者间协议.
  • 在PI-QUAL v2中,与v1.1中的"最佳"相比,PI-QUAL v2显示了向"可接受"质量的转变.
  • DCE在提高MRI质量方面发挥着至关重要的作用,作为"图像质量安全网".