血管FLRT2调节静脉介导的血管性扩张和中枢神经系统屏障的产生
C Llaó-Cid1, B Peguera1, P Kobialka1
1Buchmann Institute for Molecular Life Sciences (BMLS), Institute of Cell Biology and Neuroscience, Goethe University Frankfurt, Max-von-Laue-Str. 15, D-60438, Frankfurt am Main, Germany.
Nature communications
|November 28, 2024
概括
纤维肌菌素白丰富的转膜蛋白2 (FLRT2) 对中枢神经系统 (CNS) 静脉发育至关重要. 在小鼠中删除FLRT2会破坏视网膜静脉形成和血脑屏障的完整性.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 静脉是血管网络在发育和疾病期间扩张的内皮细胞的来源.
- 纤维素蛋白白氨酸丰富的转膜蛋白2 (FLRT2) 是一种血管性尿素,在血管发育中具有潜在的作用.
研究的目的:
- 为了研究FLRT2在小鼠中枢神经系统 (CNS) 血管发育中的功能.
- 了解FLRT2在视网膜和大脑皮层血管化以及血脑屏障形成中的作用.
主要方法:
- 在早产后小鼠中FLRT2的遗传删除.
- 视网膜血管发育的分析,包括内皮细胞的增殖,发芽和极性.
- 使用膨胀显微镜进行VE-cadherin和Numb相互作用的体内评估.
- 评估大脑皮层中血脑屏障的完整性.
主要成果:
- 删除FLRT2导致了视网膜静脉的特定缺陷,损害了内皮细胞的增殖和发芽.
- 在FLRT2突变体的血管前部观察到减少的尖端细胞.
- FLRT2与VE-cadherin和Numb相互作用,调节附着结合形态.
- FLRT2 调节粘附体和紧密结之间的交叉声,影响血脑屏障的发展.
结论:
- FLRT2是中枢神经系统血管发育的关键,静脉特异性调节器.
- FLRT2在维持血管完整性和血脑屏障功能方面发挥着关键作用.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:10Mechanism of Angiogenesis
5.3K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.3K