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Updated: Jun 6, 2025

Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
获得携带SARS-CoV-2核体保存碎片的HBV核心蛋白VLP作为疫苗候选人
Yadira Lobaina1,2, Alexis Musacchio1,2,3, Panchao Ai1,4
1Research Department, China-Cuba Biotechnology Joint Innovation Center (CCBJIC) Lengshuitan District, Yongzhou City, 425000, Hunan, China.
一种基于新型乙型肝炎核心抗原 (HBcAg) 的候选疫苗,CND-1,对SARS-CoV-2核体 (N) 蛋白产生显著的粘膜和全身免疫反应,包括与变种和SARS-CoV-1的交叉反应.
科学领域:
- 病毒学和免疫学 病毒学和免疫学
- 疫苗开发 疫苗开发
- 结构生物学 结构生物学
背景情况:
- 乙型肝炎核心抗原 (HBcAg) 作为异质蛋白碎片的载体,形成病毒样颗粒 (VLP) 并刺激免疫反应.
- 结合HBcAg与SARS-CoV-2核体 (N) 蛋白域的化学蛋白质被设计成潜在的冠状病毒疫苗候选者.
研究的目的:
- 设计和评估基于HBcAg和SARS-CoV-2 N蛋白质域作为疫苗候选者的仿制蛋白质 (CN-1和CND-1).
- 评估这些结构对SARS-CoV-2和相关冠状病毒的抗原性,免疫性和保护潜力.
主要方法:
- 在大肠杆菌中产生了重组化学蛋白 (CN-1,CND-1),并使用传输电子显微镜 (TEM) 鉴定了粒子形成的特征.
- 使用来自COVID-19康复捐赠者的血清进行了抗原性测试.
- 免疫原性在Balb/C小鼠中通过鼻内注射评估,使用或不使用CpG ODN-39 M辅助剂,随后评估系统性和粘膜性免疫反应 (抗体产生,IFN-γ ELISpot).
主要成果:
- CN-1和CND-1都形成了10-25纳米粒子,并被COVID-19康复血清识别,而CND-1显示出更高的抗原性.
- 在小鼠中,单独注射CND-1引发了针对SARS-CoV-2 N蛋白的全身和粘膜免疫反应.
- CND-1和CpG ODN-39 M配方显著增强了抗N免疫力,诱导了对Omicron变体和SARS-CoV-1的交叉反应性抗体,以及广泛的细胞免疫反应.
结论:
- 嵌合蛋白CND-1,与HBcAg融合,是第二代冠状病毒疫苗的有希望的候选人.
- 使用CpG ODN-39 M辅助剂的CND-1鼻腔配方诱导了强大的粘膜和全身免疫力,扩大了范围,超出了基于尖蛋白的疫苗.
- 这种方法提供了对Sarbecovirus N蛋白质,包括变种的广泛保护的潜力.
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