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Laurel E Redding¹, Scott G Daniel², Alexander Smith³

¹Clinical Studies - New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania, USA.

Veterinary medicine and science

|November 29, 2024

概括

在牛便中检测Clostridioides difficile (C. difficile) 的两个培养方案显示出低灵敏度,特别是在低丰度水平时. 快枪元基因组学更频繁地检测到这些细菌,这表明C. difficile流行病学研究当前培养方法的局限性.

科学领域:

兽医微生物学兽医微生物学
牛的传染病牛的传染病
分子流行病学分子流行病学

背景情况:

有效的Clostridioides difficile (C. difficile) 培养方案对于流行病学研究至关重要.
牛群中C. difficile的流行病学在很大程度上仍然没有被描述.
没有先前的研究比较了牛群中C. difficile检测试验.

研究的目的:

为了比较在牛便中检测C. difficile的两个不同的培养方案.
为了评估与猎枪元基因组测序对比的协议性能.
评估C. difficile在孕期母牛及其新生小牛中的流行情况.

主要方法:

协议1:对TCCFA基进行直接涂层丰富.
协议2:以乙醇冲击丰富,然后对CCFA/ChromID亚گار进行涂层.
这两种方案都使用了缩和非缩的配额.

主要成果:

在丰富后,C. difficile在两个小牛样本和一个母牛样本中被检测到.
检测率在协议之间没有显著差异 (p = 1.0).
快枪元基因组学在更多样本中发现了C. difficile,通常相对丰富度较低.

结论:

目前的培养方案对于在牛便中检测少量C. difficile的敏感性较低.
需要进一步优化培养方法,以对牛进行准确的C. difficile监测.
大基因组测序提供了一种更敏感的方法来检测低水平的C. difficile存在.

关键词:

无氧细菌是一种无氧细菌.细菌物种是一种细菌物种.诊断诊断诊断诊断诊断诊断

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