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在老鼠中存在的诺托金化物R₁的形式及其潜在的生物活性

Meng-Ge Feng^1,2, Lin-Han Xiang¹, Yang Li¹

¹State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.

Journal of agricultural and food chemistry

|November 29, 2024

概括

来自Panax notoginseng的notoginsenoside R1 (NG-R1) 在老鼠中经历了广泛的代谢,产生了105种代谢物,其中包括89种新型代谢物. 这些代谢物显示出潜在的抗瘤和抗炎活性,为未来的药物发现铺平了道路.

科学领域:

药理学和药理动力学药理学和药理动力学
自然产品化学自然产品化学
代谢学代谢学代谢学

背景情况:

在Panax notoginseng中的一个关键化合物,Notoginsenoside R1 (NG-R1) 是一种基于protopanaxatriol (PPT) 的素.
了解NG-R1的代谢命运对于其治疗应用至关重要.

研究的目的:

为了阐明NG-R1在老鼠中的体内代谢概况和分布.
识别新型代谢物并描述它们的代谢途径.
预测NG-R1代谢产物潜在的药理学活性.

主要方法:

超高性能液体染色学-电子喷雾电离-四极飞行时间质谱学 (UHPLC-ESI-Q-TOF-MS/MS) 用于代谢物识别.
用于代谢物表征的LC-MS数据分析.
用于活动预测的PharmMapper和网络药理学.
在体外细胞实验以验证活性.

主要成果:

确定了NG-R1的105种代谢物,其中89种是新的.
观察到新的代谢反应,包括多化,化,乙化,葡萄糖化和氨基酸结合.
在各种器官中检测到代谢物,在大肠中度最高.
预计48种代谢物具有药理活性,具有八种化合物的验证的抗瘤和抗炎作用.

结论:

NG-R1在体内经历广泛的生物转化,产生各种各样的代谢产物.
该研究提出了PPT ESI MS碎片化路径的命名法.
已识别的代谢物及其预测的活动为未来对达马兰类型三类物质的研究提供了基础.

关键词:

在UHPLC−ESI-Q−TOF−MS/MS中使用.分销销售分销销售销售销售有效的形式有效的形式.现有的 in vivo 形式已经存在.代谢产物的代谢产物不同的基因因化物R1

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