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用BacMam病毒传递牛干扰素α基因阻断病毒感染

Zhenying Fan¹, Yajuan Hou¹, Yue Liu¹

¹Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, PR China.

|November 29, 2024

概括

这项研究开发了一种新的Baculovirus介导基因传递系统 (BacMam) 用于牛干扰素-α (BoIFNα). 这种工程病毒在体外和体内都对多种牛病毒表现出强大的抗病毒活性.

科学领域:

病毒学病毒学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

干扰因子-α (IFN-αs) 是抗病毒防御的重要细胞因子.
百科病毒介导的基因转导 (BacMam) 在哺乳动物细胞中提供高效的重组蛋白表达.

研究的目的:

开发和评估用于输送牛干扰素-α (BoIFNα) 的BacMam系统.
评估表达BoIFNα的BacMam病毒对关键牛病毒病原体的抗病毒功效和机制.

主要方法:

构建一个复合的PACEMam1-BoIFNα细菌体和生成BacMam-BoIFNα病毒.
转导HEK293T和MDBK细胞,然后评估BoIFNα分泌和抗病毒活性.
在细胞培养和小鼠模型中评估病毒复制抑制,干扰素刺激基因 (ISG) 转录和M×1表达.

主要成果:

从转化细胞中成功分泌了BoIFNα蛋白,对VSV,BPIV3,BEV和BVDV表现出抗病毒活性.
该BacMam-BoIFNα病毒抑制了MDBK细胞中的病毒复制,通过JAK-STAT途径诱导ISGs和M×1表达.
在小鼠的肺和气管中观察到BPIV3复制的显著抑制.

结论:

在体外和体内,BacMam-BoIFNα病毒表现出强大的抗病毒特性.
该系统为干扰素基因传递提供了一种有效的工具,用于对抗牛病毒感染.
抗病毒机制涉及到JAK-STAT信号通路.

关键词:

在BacMam系统中,BacMam系统牛类干扰素α-α 在JAK-STAT信号通道上.抗病毒活动的抗病毒活性.病毒复制的抑制病毒复制.

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