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转录组分析揭示了IncRNA-mRNA联合表达网络,调节了海黄瓜的度

Xiaomei Chen^1,2, Wentao Han¹, Rui Yang¹

¹Fang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, 266003, China.

Marine biotechnology (New York, N.Y.)

|November 29, 2024

概括

长非编码RNAs (lncRNAs) 调节基因表达和新陈代谢在海黄aestivation. 这项研究确定了与海黄瓜有关的lncRNAs.

科学领域:

海洋生物学海洋生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

长非编码RNAs (lncRNAs) 是基因表达和代谢控制的关键调节者,影响休眠过程.
在高温期间,海黄瓜 (Apostichopus japonicus) 的低代谢策略,包括显著的生理变化,特别是在肠道和呼吸系统树.
lncRNAs在调节海黄瓜化的特定作用仍然在很大程度上未被探索.

研究的目的:

在Apostichopus japonicus中识别和表征 lncRNA.
在aestivation期间分析肠道和呼吸道树中lncRNAs的差异性表达模式.
为了阐明 lncRNAs 在海黄生长中的调节作用.

主要方法:

在A. japonicus.中鉴定和表征14711个lncRNAs.
在aestivation期间,肠道和呼吸道树组织中lncRNAs的差异表达分析.
构建 lncRNA-mRNA 同表达网络.
对保守的共同表达因子和转录因子调节的分析.

主要成果:

在A. japonicus.中,有14711个lncRNA被识别和表征.
在胃化过程中,在肠道和呼吸道树之间观察到明显的lncRNA表达模式,突出了不同的生理和代谢过程.
lncRNA-mRNA协同表达网络揭示了lncRNAs在调节aestivation期间组织特异性反应中的重要作用.
特定的lncRNAs,包括一个转换作用的lncRNA (lncrna.1393.1),被确定为关键转录因子的潜在调节者,如Klf2和Egr1,参与节律调节.

结论:

这项研究提供了A. japonicus中lncRNAs的综合目录,以及它们在化过程中的动态表达.
lncRNAs在编排海黄瓜复杂的生理和代谢适应化的过程中发挥着至关重要的作用.
这些发现提供了对休眠状态的保护性调节机制的见解,并确定了对aestivation和相关的低代谢状态的未来研究的潜在目标.

关键词:

亚波斯蒂科普斯 (Apostichopus japonicus) 是一个日本动物.这是一个估值.共同表达网络的共同表达网络长的非编码RNA是什么?节律调节节律调节节律

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