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Updated: Jun 6, 2025

Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR
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Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR

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基于RT-qPCR的一步测试的前性验证,用于量化乳腺癌中的HER2基因表达

Basma Albanyahyati1,2,3, Hicham El Hadi1, Youssef Bakri2,3

  • 1Diagnostic kit and medical devices center, Moroccan Foundation for Advanced Science Innovation and Research (MASCIR), University Mohammed VI Polytechnic, Lot 660, Hay Moulay Rachid Ben Guerir, 43150, Morocco.

Asian Pacific journal of cancer prevention : APJCP
|November 29, 2024
PubMed
概括

一个新的单步RT-qPCR测试准确量化了乳腺癌组织中的HER2表达. 该方法与FISH和IHC具有很高的一致性,为改善治疗决策提供可靠的诊断工具.

关键词:
在现场光 混合化在HER2量化过程中,乳腺癌 乳腺癌 乳腺癌免疫组织化学 免疫组织化学一个阶段RT-qPCRR

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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