和生提取物在结肠癌细胞系中的抗癌性质
Aysam M Fayed1,2, Habeeb S N2, Walaa Samy1
1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
Asian Pacific journal of cancer prevention : APJCP
|November 29, 2024
概括
这项研究表明,大和生提取物对结肠癌细胞 (Caco-2) 有效. 这些天然疗法促进了亡,减少了炎症标志物,提供了潜在的替代癌症治疗方法.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 自然产品化学 自然产品化学
背景情况:
- 大肠癌 (Caco-2) 涉及表观遗传改变,导致细胞不受控制的生长和入侵.
- 传统的治疗方法包括手术,辐射,激素治疗和化疗.
- 需要安全,具有成本效益和环保的结肠癌疗法.
研究的目的:
- 研究埃及植物提取物 (生,大,肉,香) 作为潜在的结肠癌治疗方法的疗效.
- 评估这些提取物对Caco-2细胞的细胞毒性作用.
- 探索其抗癌活性背后的分子机制.
主要方法:
- 使用高性能液体染色学 (HPLC) 和气体染色学-质谱学 (GC-MS) 分析了植物提取物.
- 用MTT测定和Annexin V-FITC流细胞计对Caco-2细胞进行了细胞毒性评估.
- 基因表达 (与自和亡相关的基因) 和蛋白质水平 (LDH,切割的PTEN,酶-3,IL-8,IL-6) 通过qRT-PCR和ELISA进行测量.
主要成果:
- 大和生提取物对Caco-2细胞表现出显著的细胞毒性.
- 这些提取物显著增加了乳酸脱酶 (LDH) 合成.
- 大和生调节了自基因 (Bectin1,Atg5,PTEN) 并通过增加切割的PTEN和酶-3来增强了亲细胞突变信号,同时降低了Atg5和Bectin1.
结论:
- 大和生提取物通过诱导细胞亡和改变关键细胞通路,表现出强大的抗结肠癌特性.
- 这些天然提取物还显著调节促炎性细胞因子 (IL-8和IL-6).
- 生和大代表了对结肠癌治疗的有希望,安全和有效的替代草药.
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