Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Uncovering hidden cross-regional environmental risks: Network evidence from off-site penalties and implications for pollution transfer in China.

iScience·2026
Same author

Author Correction: DNA nanodevices detect an acidic nanolayer on the lysosomal surface.

Nature cell biology·2026
Same author

Receptor-Tethered Cytosolic Modulators Enable Spatial Control of Cell Signaling Specificity.

ACS nano·2026
Same author

Arabidopsis Ethylene Receptors of Low Degrees of Functional Redundancy Enrich Heterogeneity in Ethylene Responsiveness.

Journal of experimental botany·2026
Same author

Immunological research landscapes and emerging immune mechanisms in HIV/HBV co-infection: a bibliometric analysis (2014-2024).

Frontiers in immunology·2026
Same author

Selection and Optimization of a High-Affinity FGFR4 Aptamer that Blocks FGF19-FGFR4 Signaling.

Analytical chemistry·2026

相关实验视频

Updated: Jun 6, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

33.4K

基于DNA体的敏感电化学生物传感器用于NAD (H) 检测.

Wenfei Guo1, Haiyuan Wang2, Zhaoyang Wang2

  • 1Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China; Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), The Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China.

Biosensors & bioelectronics
|November 29, 2024
PubMed
概括

一个新的基于aptamer的生物传感器可以准确地检测尼古丁胺-氨酸二核酸 (NAD(H),这是细胞代谢中的关键分子. 这种工具揭示了癌细胞和衰老组织的代谢差异.

关键词:
亚普特美尔 (Aptamer) 是一种药物.在DNA纳米结构中.电化学生物传感器代谢差异的不同酸 (NAD) 酸 (H) 是一种

更多相关视频

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.0K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.0K

相关实验视频

Last Updated: Jun 6, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

33.4K
A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.0K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.0K

科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 分子生物学分子生物学

背景情况:

  • 尼古丁胺-腺因二核酸 (NAD) 对于细胞代谢至关重要,了解其水平对于疾病研究至关重要.
  • 开发用于NAD (H) 检测的特定探针和敏感生物传感器仍然是一个重大的科学挑战.

研究的目的:

  • 开发一种高度选择性和敏感的电化学生物传感器,用于准确的NAD (H) 检测.
  • 选和识别具有对NAD (H) 的特定结合亲和力的阿马体.

主要方法:

  • 选的阿普坦体以识别特定的NAD (H) 结合剂,以NAD3-1a为例,具有微分子亲和力.
  • 选择的阿普坦与四面体DNA纳米结构的集成,以创建一个电化学生物传感器.
  • 验证生物传感器的性能,包括其线性检测范围,稳定性和可重复性.

主要成果:

  • 开发的以aptamer为基础的生物传感器表现出高选择性和对NAD (H) 检测的灵敏度,在线性范围内为10−12到10−7M.
  • 生物传感器在测量中表现出极好的稳定性和可重复性.
  • 在正常和瘤细胞之间观察到NAD (H) 水平的显著变化.
  • 在老年小鼠的骨肌组织中检测到NAD (H) 水平的显著降低.

结论:

  • 基于aptamer的电化学生物传感器为精确的NAD (H) 量化提供了一个强大的工具.
  • 这种生物传感器有可能在各种生理和病理条件下显著提高对代谢失调的理解.
  • 这些发现强调了阿普坦-DNA纳米结构合体在开发关键生物分子的先进生物传感平台方面的实用性.