工程细胞膜覆盖的金属有机框架纳米晶体用于细胞内货物输送
Martina Migliavacca1, Manuela Cedrún-Morales1, Manuel Ceballos1
1Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Física de Partículas, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, Spain.
Journal of colloid and interface science
|November 29, 2024
概括
研究人员开发了细胞膜涂层的纳米金属有机框架 (NMOFs),用于向细胞内药物输送. 这种仿生方法增强了细胞吸收和货物释放,显示了癌症治疗的前景.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 药物输送系统 药物输送系统
背景情况:
- 金属有机框架 (MOF) 为药物封装提供可调节的多孔性.
- 细胞膜覆盖增强了纳米粒子的生物相容性和向性.
- 细胞内传递面临着诸如内分泌体捕获等挑战.
研究的目的:
- 开发和描述细胞膜覆盖的UiO-67纳米金属有机框架 (NMOFs).
- 评估这些NMOF在模型货物的细胞内输送方面的有效性.
- 评估这个平台在向癌症药物供应方面的潜力.
主要方法:
- 合成和功能化的UiO-67 MOFs.
- NMOFs被来自A549细胞的融合细胞膜衍生的纳米纤维 (FCSMs) 覆盖.
- 量化了克雷西尔紫色 (CV) 和碳白 (CbPt) 的加载和释放.
- 用2D和3D细胞模型评估了细胞吸收和递送效率.
主要成果:
- 被细胞膜覆盖的NMOF证明了有效的货物加载和持续释放.
- 生物仿真涂层促进了增强的细胞吸收和直接的细胞质递送.
- 成功避免了内分泌体捕获.
- 在2D培养和3D球形体中观察到CV和CbPt的有效传递.
结论:
- 具有细胞膜遮的工程NMOF提供了一个有前途的平台,用于增强细胞内药物输送.
- 仿生方法提高了生物相容性,并使有针对性的交付成为可能.
- 这一策略有可能提高癌症治疗中的化疗疗效.
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