高葡萄糖通过累积效应增强MDA-MB-231细胞的恶性进展
Gaotao Zhang1, Zhiqin Liu2, Huixin Zheng1
1College of Life Science, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China.
Toxicology letters
|November 29, 2024
概括
高血糖水平通过影响细胞增殖和炎症因素促进三阴性乳腺癌 (TNBC) 的进展. 这项研究揭示了参与高葡萄糖诱导的TNBC发展的关键分子通路,为2型糖尿病 (T2DM) 管理提供了洞察力.
科学领域:
- 在瘤学瘤学.
- 代谢疾病 代谢疾病
- 分子生物学分子生物学
背景情况:
- 高葡萄糖与乳腺癌的进展有关,但其对三阴性乳腺癌 (TNBC) 的具体影响尚未完全理解.
- 2型糖尿病 (T2DM) 是一种普遍的疾病,对癌症的发展和进展有潜在的影响.
研究的目的:
- 研究短期和长期高葡萄糖暴露对MDA-MB-231 TNBC细胞系的分子效应.
- 确定TNBC中高葡萄糖影响的关键信号通路和基因.
- 阐明高葡萄糖对TNBC细胞增殖,炎症和血管生成的影响.
主要方法:
- RNA测序 (RNA-seq) 用于在短期和长期高葡萄糖条件下分析TNBC细胞中的基因表达变化.
- 生物信息分析用于识别差异表达基因 (DEG) 和枢纽基因.
- 进行了体外实验,以评估细胞增殖,炎症因子积累和血管生成.
主要成果:
- 总共有896个DEG被确定,其中57个在短期和839个在长期高葡萄糖组中.
- 短期高葡萄糖主要影响IL-17信号通路,而长期暴露影响IL-17,MAPK,TNF,AGE-RAGE,Toll-like受体和VEGF信号通路.
- 高葡萄糖促进了TNBC细胞增殖,具有时间依赖的效果,诱导炎症因子积累,并增强了血管生成.
结论:
- 高葡萄糖的微环境通过各种分子途径显著影响TNBC的进展.
- 与短期暴露相比,长期高葡萄糖暴露对TNBC基因表达和信号通路产生更深远的影响.
- 这些发现突显了T2DM和TNBC之间的复杂关系,表明了潜在的治疗点.
相关概念视频
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
Adaptive Mechanisms in Cancer Cells
5.7K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.7K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Tumor Progression
6.2K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.2K
Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
Hormones Regulating Blood Glucose
3.1K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
3.1K


